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Identifier 000382627
Title Μελέτη της παραγωγής βιο-υδρογόνου (Η2) από το μικροφύκος Chlamydomonas reinhardtii
Alternative Title Biohydrogen (H2) production study from the microalgae Chlamydomonas reinhardtii
Author Μίχογλου Στεργίου, Αριστείδης
Thesis advisor Γανωτάκης, Δημήτριος Φ.
Abstract Concerns about energy supply, combined with global warming and environmental pollution, makes it imperative to develop alternative energy sources that are clean, renewable and environmentally friendly. Hydrogen is thought to be ideal as an alternative fuel. It has a high energy content since, compared to traditional hydrocarbon fuels, can generate roughly three times more energy through its combustion. Moreover, hydrogen is characterized as the cleanest energy source, since the sole product of its combustion is water. Photosynthetic hydrogen production presents a promising method for the development of a new economically advantageous industrial production. The main objective of this master thesis was the study of the hydrogen production mechanism by sulfur deprived Chlamydomonas reinhardtii. Although in the presence of sulfur green algae perform normal photosynthesis, in the absence of sulfur photosynthetic activity is inhibited and the algae slip into the Η2-production mode, resulting in the generation of significant amounts of Η2 gas, essentially by using sunlight and water. So far, sulfur deprivation has been proven the most efficient method; the impact on the photosynthetic apparatus, especially on Photosystem II, is very important for the entire process. The role of Photosystem II in hydrogen production has been explored by using various mutants that have differences in critical phases of the photosynthetic process, resulting to a different behavior of PSII. In addition to whole cells, various photosynthetic preparations such as chloroplasts, thylakoids and Photosystem II enriched membranes were also investigated. Specifically, their activity and their protein composition were thoroughly examined. The main conclusion of these studies is that for effective hydrogen production an active Photosystem II is necessary. In addition, the presence of a hydrogen production mechanism, which does not require sulfur deprivation is also present in Chlamydomonas. The second part of this thesis is related to the assembly of a hydrogen photoproducing biosystem. Thylakoid membranes combined with a NiFe-Hydrogenase from bacillus Ralstonia paucula are able to catalyze the crucial steps in a biological energy conversion, generating hydrogen by using water and light. The use of light and water as an electron donor are the key-points, which could be used for the low cost production of biohydrogen.
Language Greek
Subject Sulfur deprivation
Στέρηση θείου
Issue date 2014-03-28
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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