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Title Αφαίρεση βιολογικών επικαθίσεων με χρήση laser και αξιολόγηση της αποτελεσματικότητάς της μέσω φασματοσκοπίας φθορισμού επαγόμενου από LED (LED-induced fluorescence)
Alternative Title Laser-assisted removal of stone biodeterioration and evaluation of its effectiveness by LEDinduced fluorescence spectroscopy
Author Σταμούλη, Νεφέλη
Thesis advisor Πουλή, Παρασκευή
Reviewer Ρακιτζής, Πέτρος
Άγγλος, Δημήτριος
Σαμαρτζής, Πέτρος
Abstract The present work is a study of the laser cleaning methodology of a biodeteriorated mural surface. The cleaning tests took place on a mural fragment from a church located in Cyprus. In order to detect the biological components on the fragment’s surface, a portable device that induces fluorescence through an LED source was used. The fluorescence spectra (LED – IF) were able to provide semi-quantitative information on the presence of fluorophores before cleaning. The experimental conditions for spectrum acquisition were explored, considering both LED sources (440 nm and 375 nm) and measurements on dry and wet surfaces. These preliminary studies aimed to determine optimal conditions that balance stability and objectivity in measurements. Fluorescence, being a dynamic process influenced by photosynthetic organisms adapting to external conditions, posed challenges in quantification. Laser cleaning experiments were conducted using infrared (1064 nm), visible (532 nm), and combined wavelengths. The evaluation of cleaning effectiveness was based on the development of the R ratio, incorporating fluorescence signals from chlorophyll and the substrate after cleaning. Python programming language was utilized for data processing, fitting a multi-peak fluorescence model to isolate signal values from chlorophyll and the substrate. The R ratio allowed for the comparison of cleaning efficiency across different points, categorizing them into effective, partially effective, and ineffective cleaning outcomes.
Language Greek
Issue date 2024-03-29
Collection   School/Department--School of Sciences and Engineering--Department of Physics--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/9/8/c/metadata-dlib-1708936820-222522-16628.tkl Bookmark and Share
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