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Identifier |
000443249 |
Title |
Ανάπτυξη και εφαρμογή πηγών ιοντισμού υπερηχητικής εκνέφωσης με φασματομετρία μάζας για την ανάλυση περιβαλλοντικών και βιολογικών δειγμάτων |
Alternative Title |
Development and application of sonic spray ionization sources for mass spectrometric analysis of environmental and biological samples |
Author
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Γραφανάκη, Σοφία Ν.
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Thesis advisor
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Περγαντής, Σπυρίδων
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Reviewer
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Απόστολος, Σπύρος
Λυδάκης-Σημαντήρης, Νικόλαος
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Abstract |
The technique of Sonic Spray Ionization Mass Spectrometry (SSI-MS) was the
basis of the development and application of two analytical methods, Orthogonal
Sonic spray Ionization (Ortho-SSI) and Desorption sonic spray Ionization Mass
Spectrometry (DeSSI-MS). The development of each ionization source involves the
selection of optimum parameters before the analysis of real samples.
The first part consists of the detailed development of the Orthogonal Sonic spray
Ionization source. That involves the interaction of a high velocity spray with a
cylindrical rode that bends the spray from a SSI nebulizer and thus introduces SSI –
originating droplets into the MS in order to improve the efficiency with which high
performance liquid chromatography (HPLC) can be coupled to SSI-MS. The HPLCOrtho-SSI-MS/MS was used to separate and detect chlorate (ClO3-), perchlorate
(ClO4-) and bromate (BrO3-) ions and quantitate these anions in water samples.
In the second part, Chlorfenapyr insecticide metabolism was investigated for four
recombinant proteins of the cytochrome p450 from Anopheles gambiae and Αedes
aegypti mosquitoes, which associated with insecticide resistance. The technique of
HPLC-ESI-MS was used for this analysis, and the response of the system was
compared to HPLC-Ortho-SSI-MS. Only one of the three enzymes was not able to
metabolise this insecticide.
In the last section, Easy Ambient Sonic Spray Mass Spectrometry (EASI or
DeSSI-MS) was developed and applied for the rapid analysis of C. reinhardtii cells
incubated with sub lethal concentrations of selenium. The main characteristic is that
minimal sample preparation is needed for the analysis of cells. Membrane lipids such
as phosphatidylglycerol (PG), sulfoquinovosyldiacylglycerol (SQDG) and 1,2-
diacylglyceryl-3-O-4’-(N,N,N- trimethyl)-homoserine (DGTS) were detected. The
effect of toxic metalloids [Se(IV) και Se(VΙ)] resulted in different profiles of lipids in the
mass spectra that possibly indicate membrane lipid double-bond reduction.
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Language |
Greek |
Subject |
High performance Liquid Chromatography |
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Mass spectrometry |
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Ιοντισμός υπερηχητικής εκνέφωσης |
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Υγρή χρωματογραφία υψηλής απόδοση |
Issue date |
2021-11-24 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/4/e/8/metadata-dlib-1635761554-46174-14791.tkl
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Views |
272 |