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Identifier

000429199

Title

Χαρακτηρισμός ενός στελέχους Chlorella που απομονώθηκε από ποταμό στην Κρήτη και πιθανές εφαρμογές στη βιομηχανία τροφίμων και διαχείριση αποβλήτων

Alternative Title

Characterization of a Chlorella strain isolated from a river in Crete and possible applications in food industry and waste management

Author

Στρατηγάκης, Ναπολέων-Χριστόφορος Μ.

Thesis advisor

Γανωτάκης, Δημήτριος

Reviewer

Κοτζαμπάσης, Κυριάκος
Παυλίδης, Ιωάννης

Abstract

The present study focuses on the characterization of a novel Chlorella strain isolated from a eutrophic site at Giofyros River (Heraklion, Crete, Greece). The aim of this study was to evaluate this microalga for applications in the bioremediation of rich in organic matter industrial waste waters. The first part of this thesis focuses on the genetic characterization of the strain, the study of a broad range of properties related to cell growth rate, as well as its resistance to common chemical inhibitors. 18S rRNA gene analysis as well as the Neighbor- joining phylogenetic tree inferred from partial 18S rRNA gene sequence, showed that this microalga belongs to the Chlorella genus. Photoheterotrophically grown cells achieved remarkably high growth rates, despite the low light intensities used in the experiments; the photosynthetic rate under variouslight conditions was assessed as well. Higher levels of photosynthetic activity were observed for photoautotrophic cells, while photoheterotrophic cells were more tolerant to higher light intensities. Photoautotrophic cells were found to contain higher amounts of carbohydrates and photosynthetic pigments, while their photoheterotrophic counterparts showed a slightly elevated lipid content. Under both growth conditions, the cells did contain similar protein levels. Furthermore, the strain was found to be particularly resistant to the deleterious effects of chemical inhibitors, even at the relatively high concentrations tested. This ability was attributed to an extensive extracellular polysaccharide matrix, which seems to play a protective role for cell homeostasis maintenance. In the second part of this thesis, the ability of the isolated Chlorella strain to grow in dairy waste-waters was investigated. The cells were able to acclimate to the wastewater media; however the growth was limited due to the lack of appropriate nutrients. Thus, the enrichment of dairy waste-waters with low amounts of nutrients allowed higher cell growth rates. Moreover, the bioremediation of the waste-waters by the microalga was evaluated through the removal of sugars in the media. Chlorella cells grown in media supplemented with dairy waste were found to have biochemical content similar to the one observed with photoautotrophic grown cells.

Language

Greek

Subject

18S rRNA gene

Bioremediation

Dairy wastewaters

Growth

Microalgae

Photosynthesis

Photosynthetic activity

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