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Identifier

000397208

Title

Μοριακή ανάλυση της κυτταροπλασματικής ασυμβατότητας που προξενεί η Wolbachia στη Μεσογειακή φρουτόμυγα με χρήση πρωτεϊνωματικής

Alternative Title

Molecular analysis of the cytoplasmic incompatibility caused by Wolbachia in mediterranean fruitfly using comaparative proteomics

Author

Κουντουράκης, Νικόλαος Σ.

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Πανεπιστήμιο Κρήτης

Thesis advisor

Οικονόμου, Αναστάσιος

Abstract

The Mediterranean fruit fly (Medfly), Ceratitis capitata is considered one of the most destructive pests of fresh fruit and vegetables worldwide. It can infest more than 300 plant species and exhibits today an almost global distribution. Medfly population control is based mainly on synthetic insecticides which is very costly and environmental harmful. Projects operating at national and international level have been implemented in several regions all over the globe to suppress or eradicate Mediterranean fruit fly populations. These programs rely on the release of high numbers of mass-reared, sterilized males (Sterile Insect Technique, SIT). Mass rearing, irradiation and handling conditions may affect several behavioural traits of the produced males, diminishing their ability to compete with wild males to mate with wild females. To compensate the reduced fitness more males need to be released, thus raising the cost of the suppression program. Wolabchia, an intracellular endosymbiont bacterium, is widely distributed among arthropods and nematodes. It mainly localizes in host reproductive tissues and organs and manipulates host reproduction through the following mechanisms: parthenogenesis, feminization, male killing, cytoplasmic incompatibility (CI). CI is expressed as embryonic lethality in crosses between a Wolbachia-infected male and a female of different infection status. Given that Wolbachia transmission is directly related to survival and reproduction of its hosts, it is expected that Wolbachia infections would increase the fitness of host populations. Based on the above, Wolbachia-based strategies are promising approaches to the population control of insect pests such as Mediterranean fruit fly. The scope of this study is to determine the alterations in protein level that causes the MedFly/Wolbachia symbiosis. More precisely, a comparative proteomics analysis testes and ovaries of Medfly-V8, in the presence or absence of Wolbachia will be performed to identify proteins that are involved in cytoplasmic incompatibility. The effect of Wolbachia on insects has been studied in general with very few proteomics tools and no insect/symbiotic bacterium system has been analyzed in any depth.

Language

Greek

Subject

Symbiomics

Πρωτεϊνωματική ανάλυση

Συμβίωση

Issue date

2015-11-20

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

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