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Identifier

000381647

Title

Ανάπτυξη μεθόδων καταπολέμησης εχθρών των καλλιεργειών με RNA σίγηση (πειράματα με αλευρώδη και σίγηση μονοπατιού για αποτοξικοποίηση)

Alternative Title

Development of novel insect pest-control strategies using RNA silencing, a case study of silencing the oxidative metabolic pathway in whiteflies (Bemisia Tabaci)

Author

Σιώζιου, Ελένη

Thesis advisor

Βόντας, Ιωάννης

Abstract

RNA silencing is a conserved mechanism found in most eukaryotes. It is triggered by the presence of double stranded RNA molecules which guide the suppression of complementary sequences. It acts either at the transcriptional level or at the post-transcriptional level. The last years the use of RNAi is suggested as a novel strategy for insect pest-control, via silencing biologically important insects’ genes. The main methods investigated for the delivery of dsRNA are either by spraying directly to the plants or by construction of transgenic plants which express dsRNA. At this project we tried to develop a novel RNAi based technique for silencing the oxidative metabolic pathway of Bemisia tabaci. We tried two approaches: a) oral delivery through artificial diet by in vitro synthesis of dsRNA for the proteins CYP6CM1 and CPR and b) production of transgenic plants Nicotiana tabacum that express dsRNA for CPR protein.

Language

Greek

Subject

Dependent monooxygenase

Detoxification

Nicotiana Tabacum

P450s

Οξειδάσες

Issue date

2013-11-15

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses