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Identifier |
000385961 |
Title |
Ανάπτυξη μεθόδου για τον προσδιορισμό των μεταβολιτών των Παραβενίων και της Δισφιανόλης Α στα ούρα με χρήση υγρής χρωματογραφίας-δίδυμης φασματομετρίας μάζας |
Alternative Title |
Method development for the determination of Parabens and Bisphenol-A metabolites in human urine by high pressure liquid chromatography-tandem mass spectrometry |
Author
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Μπαλασκά Τσαμπίκα-Ειρήνη
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Thesis advisor
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Στεφάνου, Ευριπίδης
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Reviewer
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Κατερινόπουλος, Χαράλαμπος
Περγαντής, Σπυρίδων
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Abstract |
Endocrine disruptors (EDs) can interfere with the hormone system in mammals. Scientific research has demonstrated many compounds which act as endocrine disruptors that have endocrine activity. Exposure to endocrine disruptors can occur through skin, ingestion and via the respiratory tract. People are more vulnerable to these chemicals, through fetal life and childhood.
Bisphenol-A (BPA), is used mainly as plasticizers and it has been associated with some problems due to its endocrine activity. It is excreted in urine unmetabolized or as its glucuronide and the sulphate conjugates. Parabens (PBs), are used as antimicrobial agents. These compounds exhibit toxicity and they are linked with breast cancer in humans. Studies determine that they are excreted in urine in their original form, as a para-hydroxy-benzoic acid ( PHBA) or as its glycine, glucuronic and sulfate conjugates. The total metabolites of BPA and PBs are biomarkers of the exposure to these chemicals.
The aim of this study is the modification of an existing analytical method for the determination of phthalate metabolites in human urine, in order to also detect BPA and PBs. Emphasis was given to the separation of the two isomers of propyl paraben (PPB) and butyl paraben (BPB). The method was applied to real urine samples, obtained from 80 men.
The applied method uses solid phase extraction (SPE) and liquid chromatography coupled with tandem mass spectrometry with electrospray ionization source (HPLC-ESI-MS/MS). The method detection limits were from 0,04 ng/mL to 0,13 ng/mL for PBs and 0,07 ng/mL for BPA, the recoveries were satisfactory, blanks were zeros and the repeatability was very good.
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Language |
Greek |
Subject |
APCI |
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ESI |
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Endocrine disruptors |
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HPLC |
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MS |
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Ενδοκρινικοί διαταράκτες |
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Ηλεκτροψεκασμός |
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Χημικός ιονισμός ατμοσφαιρικής πίεσης |
Issue date |
2014-07-25 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/b/2/9/metadata-dlib-1405685702-881564-28794.tkl
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Views |
245 |