E-Locus - Institutional Repository of the University of Crete

Home Search

Results - Details

Search command : Author="Λυδάκης" And Author="Σημαντήρης" And Author="Νικόλαος"

Current Record: 5 of 6

[Add to Basket]

Identifier

000419456

Title

Development and application of desorption sonic-spray ionization mass spectrometry for the direct analysis of membrane lipids in microorganism cells

Alternative Title

Ανάπτυξη και εφαρμογή φασματομετρίας μάζας εκρόφησης με ιοντισμό υπερηχητικής εκνέφωσης για την απ' ευθείας ανάλυση μεμβρανικών λιπιδίων σε κύτταρα μικροοργανισμών

Author

Μαυρουδάκης, Λεωνίδας Κ.

Thesis advisor

Περγαντής, Σπύρος

Reviewer

Γανωτάκης, Δημήτρης
Λυδάκης-Σημαντήρης, Νικόλαος

Abstract

In this thesis, Desorption Sonic-Spray Ionization Mass Spectrometry (DeSSI-MS) was developed and applied for the rapid analysis of C. reinhardtii CC-1690 and Synechocystis sp. PCC 6803 cells under different growth conditions. The sample preparation of the cells was minimal and only involved the washing of cells with water to remove media components. No extraction protocols were used prior to mass spectrometric analysis of these cells. Under these conditions, mass spectra in positive and negative ion mode were obtained and membrane lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), phosphatidylglycerol (PG), sulfoquinovosyldiacylglycerol (SQDG), phosphatidylinositol (PI) and 1,2-diacylglyceryl-3-O-4’-(N,N,N- trimethyl)-homoserine (DGTS) were detected. The effect of toxic metals (Cd(II), Pb(II)) and metalloids (As(III), As(V)) under various concentration on membrane lipids of C. reinhardtii cells was studied and the obtained results indicate possible membrane lipid reorganization and reduction induced by these metals. Also, the effect of nitrogen deprivation on Synechocystis sp. PCC 6803 cells was investigated and it was found that physiological conversion of membrane lipids from more unsaturated to less unsaturated was disrupted, however, upon resuspension in N-containing medium the cells culture was able to return to its normal state. To the best of our knowledge this is the first time that DeSSI-MS was applied for membrane lipid analysis of cells grown under different stress conditions.

Language

English

Subject

Cyanobacteria

Desorption sonic-spray ionization

Green algae

Mass spectrometry

Εκρόφηση με ιοντισμό υπερηχητικής εκνέφωσης

Κυανοβακτήρια

Μεμβρανικά λιπίδια

Πράσινα άλγη

Φασματομετρία μάζας