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Ανάπτυξη και εφαρμογή αναλυτικών τεχνικών φασματομετρίας για τον ποσοτικό προσδιορισμό σεληνίου και των μορφών του στα τρόφιμα
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| Identifier | 000328556 | |||
| Title | Ανάπτυξη και εφαρμογή αναλυτικών τεχνικών φασματομετρίας για τον ποσοτικό προσδιορισμό σεληνίου και των μορφών του στα τρόφιμα | |||
| Alternative Title | Development and application of mass spectrometry analytical techniques for the quantitative determination of selenium and selenium species in food products | |||
| Author | Τσιάμουρου, Ιωάννα | |||
| Thesis advisor | Περγαντής, Σπύρος | |||
| Abstract |
Aiming at the speciation of selenium species were developed various methods of extraction (hydrolysis extraction, extraction with buffer, enzymatic extraction), in two kinds of fish, for the suitable method that will give the better desirable results and concrete, the higher recovery of the extraction. The higher recovery of the extraction has been found when we used the enzymatic digestion with the addition of enzyme Pronase E. Also an in vitro gastrointestinal method was accomplished for the determination of % bioavailability of selenium, the quantity of selenium which is absorbed by the human organism, when he consumed the two kinds of fish. In vitro gastrointestinal digestion includes a simulated gastrointestinal digestion with the pepsin in gastric stage (juice) and a mixture of pancreatin, bile salts and a-amylase at the intestinal stage (juice). The extracts of the enzymatic and gastrointestinal digestion have been analyzed with HPLC-ICP-MS (reversed phase chromatography). The results of speciation analysis proved that the dominant form of selenium in the two kinds of fish is selenomethionine (SeMet). |
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| Language | Greek | |||
| Issue date | 2008-06-24 | |||
| Collection | School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses | |||
| Type of Work--Post-graduate theses | ||||
| Views | 216 | |||
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