|
Identifier |
000448423 |
Title |
Μελέτη αποφυλλοποίησης και επαναστοίβαξης ανόργανων μητρών LDH (Στοιβαγμένων Διπλών Υδροξειδίων) με χρήση στη στόχευση φαρμάκων |
Alternative Title |
A study of the exfoliation and restacking of LDH (Layered Double Hydroxide) inorganic matrices with use in drug targeting |
Author
|
Σφενδουράκης, Γεώργιος - Παναγιώτης Κ.
|
Thesis advisor
|
Γανωτάκης, Δημήτριος
|
Reviewer
|
Τσιώτης, Γεώργιος
Χανιωτάκης, Νικόλαος
Βαμβακάκη, Μαρία
Παυλίδης, Ιωάννης
Σπύρος, Απόστολος
Ελευθεριάδης, Νικόλαος
|
Abstract |
The aim of this thesis was the synthesis of bioinorganic complexes, developed
through the entrapment of organic substances inside layered double hydroxides. A
plethora of molecules with biologic applications was examined, with emphasis on amino
acids and deoxyribonucleic acid, while at the same time alterations to the inorganic matrix
with the purpose of improving its capacity were investigated.
The successful synthesis of layered double hydroxides, composed of magnesium
and aluminum in a 2 to 1 ratio, is described. The inorganic matrix was then used to entrap
anions of ascorbate, lactate, acetate and cholate, as well as the drug nitrofurantoin. These
nanobiohybrids were characterized by x-ray diffraction (XRD) and infrared spectroscopy
(IR).
The exfoliation of layered double hydroxides through two new methods was
examined, more specifically through sonication of the matrix dissolved in glycerol or
propyleneglycol. These methods were subsequently compared to the most efficient
already established methods of exfoliation. The exfoliation was confirmed through
dynamic light scattering and liquid x-ray diffraction (liquid XRD), and was followed by
examination of its stability, which showed that the new solvents have a better exfoliation
profile than those established in the literature.
The above techniques were then used to insert molecules inside layered double
hydroxides through restacking. This method was investigated as an alternative to the
expensive coprecipitation, which is widely used to insert molecules, such as amino acids.
By using restacking, 16 LDH nanobiohybrids containing amino acids were then
synthesized and compared to similar ones prepared by anion exchange. The results were
compared to those in the literature, and it was demonstrated that restacking has
advantages over other methods of synthesizing LDH nanobiobrids and could lead to
successful synthesis in experiments where anion exchange could not be used. In
addition, through these experiments, problems in the purification processes that are
usually developed during LDH synthesis are highlighted, emphasizing the importance of
the medium used during the purification process and how this affects the final
nanobiobrid.
Finally, the advantage of the increased active surface of the exfoliated LDH and
subsequent restacking was examined in order to insert molecules that cannot easily be
inserted because of their size, such as deoxyribonucleic acid.
|
Language |
Greek |
Subject |
Acetic acid |
|
Amino acids |
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Ascorbic acid |
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Cholic acid |
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Deoxyribonucleic acid (DNA) |
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Drug delivery |
|
Lactic acid |
|
Layered double hydroxides (LDH) |
|
Nanobiohybrids |
|
Nitrofurantoin |
|
Αμινοξέα |
|
Αποφυλλοποίηση |
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Ασκορβικό οξύ |
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Γαλακτικό οξύ |
|
Δεοξυριβονουκλεϊκό οξύ (DNA) |
|
Επαναστοίβαξη |
|
Μεταφορά φαρμάκων |
|
Νανοβιοϋβρίδια |
|
Νιτροφουραντοΐνη |
|
Οξικό οξύ |
|
Στοιβαγμένα διπλά υδροξείδια (LDH) |
|
Χολικό οξύ |
Issue date |
2022-06-07 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Chemistry--Doctoral theses
|
|
Type of Work--Doctoral theses
|
Views |
342 |