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Identifier

000383363

Title

Απομόνωση και χαρακτηρισμός βακτηριοφάγων για την καταπολέμηση της δονακίωσης στην υδατοκαλλιέργεια

Alternative Title

Isolation and characterization of bacteriophages against vibriosis in aquaculture

Author

Καλατζής, Παναγιώτης Γ.

Thesis advisor

Παυλίδης, Μιχαήλ

Reviewer

Κεντούρη, Μαρουδιώ
Καθάριος, Παντελής

Abstract

The goal of this work was the isolation and characterization of lytic bacteriophages against fish pathogenic Vibrios. A pathogenic Vibrio sp. common in the facilities of s of HCMR which was isolated during a vibriosis outbreak in Sparus aurata was used as a host. The bacterial strain was identified as Vibrio alginolyticus, following biochemical and molecular characterization. Water from various sources and mussel from vibriosis-affected fish farms were sampled to isolate phages. Samples were enriched with nutrients as well as with the bacterial host and after filtration and purification procedures, 7 monoclonal bacteriophages were isolated. After increasing the titer of the 7 clonal phages, their morphology, host range and genome were studied. The 7 clonal phages following characterization were assigned into two different groups. One representative phage of each group, St2 and Grn1, were selected for further identification of their life cycle biological features (lytic activity, latency period, burst size and adsorption rate). Efficacy of newly isolated bacteriophages was studied under both in vitro and in vivo experimental conditions. In vitro: By infecting bacterial host’s cultures, intense lytic activity was observed by diminishing the bacterial population to almost zero optical density for more than 6 hours. In vivo: An effective phage cocktail was composed and added to live feeds cultures of Brachionus plicatilis resulting to the successful limitation of their bacterial load of Vibrio. Current study’s results conclude that bacteriophages can be used efficiently to control the populations of Vibrio that enter the marine hatcheries through live feeds.

Language

Greek

Subject

Bacteria

Phage therapy

Vibrio

Βακτήρια

Βακτηριοφάγοι

Ιsolation

Φαγοθεραπεία

Issue date

2014-03-28