Post-graduate theses
Current Record: 12 of 802
|
Identifier |
000458934 |
Title |
Akt1 depletion in neonatal macrophages protects from Group B Streptococcus infection via Slamf8-dependent autophagy |
Alternative Title |
Η έλλειψη της κινάσης Akt1 στα νεογνικά μακροφάγα προστατεύει από τη μόλυνση του Στρεπτοκόκκου τύπου Β μέσω αυτοφαγίας εξαρτώμενης από τον υποδοχέα Slamf8 |
Author
|
Χατζησεβαστός, Στέργιος Ι
|
Thesis advisor
|
Τσατσάνης, Χρήστος
Βεργαδή, Ελένη
|
Abstract |
Group B Streptococcus (GBS) is a commensal bacterium that can become highly invasive,
posing a threat to vulnerable populations. Neonates are especially at risk, facing severe
infections. Despite screening and antibiotics for pregnant women, neonatal transmission
remains a concern. Developing a universal GBS vaccine is challenging, making it crucial to
identify new protective measures. During infancy, the adaptive immune system is
underdeveloped, relying heavily on innate immunity. Macrophages, key innate immune cells,
recognize threats through receptors and polarize into pro-inflammatory (M1) or antiinflammatory (M2) states. Akt kinases play a role in this process. In the presence of pathogens,
macrophages tend to become M1, enhancing their ability to kill bacteria. Autophagy also aids
in pathogen elimination and antigen presentation, boosting the body's defense.
Akt1-deficient neonatal macrophages showed enhanced bactericidal capacity against GBS,
likely due to their M1-like pro-inflammatory profile. They exhibited increased colocalization of
LC3-II, an autophagy component, with GBS, suggesting an elimination pathway. RNA
sequencing revealed high Slamf8 receptor expression in Akt1-deficient macrophages, and
knocking down Slamf8 reduced bacterial clearance. This receptor promoted LC3-positive
phagosome formation in Akt1-deficient cells. This study enhances our understanding of
neonatal macrophage responses to bacterial infections and may lead to novel therapeutic
targets for neonatal infectious diseases.
|
Language |
English |
Subject |
Akt1 kinase |
|
Akt1 κινάση |
|
GBS |
|
SLAMF receptors |
|
Υποδοχείς SLAMF |
Issue date |
2023-11-24 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
|
|
Type of Work--Post-graduate theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/4/3/c/metadata-dlib-1695900488-708829-14050.tkl
|
Views |
1206 |
Digital Documents
|
|
No permission to view document.
It won't be available until: 2026-11-24
|