Graduate theses
Current Record: 16 of 246
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Title |
Μελέτη της παραγωγής βιοϋδρογόνου από ένα αζωτοδεσμευτικό βακτήριο σε διαφορετικά υποστρώματα σακχάρων |
Alternative Title |
A study of biohydrogen production by a nitrogen-fixing bacterium under different carbon substrates |
Author
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Τσιχλάκη, Αλεξάνδρα Αλεξία
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Thesis advisor
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Γανωτάκης, Δημήτριος
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Abstract |
In this thesis, the effect of various growth conditions on the cell growth and hydrogen
production by the nitrogen-fixing bacterium Clostridium pasterianum was investigated. This
bacterium has the ability to produce biohydrogen through the process of dark fermentation,
which is an environmentally friendly fuel that can help reduce environmental pollution.
Initially, the effect of the inoculum size was studied, as well as the growth of the
microorganism in nutrient media with different carbon and nitrogen substrates. The above
parameters had a significant effect on hydrogen production and cell growth. Next, the growth
and hydrogen production were studied under different sugar substrates, as well as their
concentrations. Cultures of the bacterium grown on mannitol, sorbitol, glucose and fructose
showed the highest hydrogen production, even at the lowest sugar concentrations, while on
disaccharides such as maltose and sucrose, the production was comparatively lower. In
addition, hydrolyzed cheese whey with high organic load was used as a growth medium for the
bacterium. According to the results, the microorganism showed both cell growth and hydrogen
production at close levels to the control cultures.
From the results of this study, it was concluded that the rational design of growth
parameters can optimize biohydrogen production during the dark fermentation by the
bacterium. Furthermore, the use of industrial wastewaters, such as cheese whey, as a growth
media allows the sustainable production of biofuels with lower economic costs
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Language |
Greek |
Subject |
Carbon source |
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Clostridium pasteurianum |
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Dairy wastewaters |
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Dark fermentation |
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Growth |
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Hydrogen |
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Nitrogen-fixing bacteria |
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Αζωτοδεσμευτικά βακτήρια |
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Ανάπτυξη |
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Απόβλητα τυροκομείου |
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Πηγή άνθρακα |
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Σκοτεινή ζύμωση |
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Υδρογόνο |
Issue date |
2024-03-21 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/2/e/5/metadata-dlib-user1710172548-12813.tkl
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Views |
253 |