|
Identifier |
000466079 |
Title |
Προσδιορισμός αρσενολιπιδίων σε τέσσερα είδη ψαριών του Αιγαίου |
Alternative Title |
Determination of arsenolipids in four fish species of the Aegean sea |
Author
|
Δρακωνάκη, Χρυσοβαλάντω - Μαρία Ν.
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Thesis advisor
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Περγαντής, Σπυρίδων
|
Reviewer
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Σπύρος, Απόστολος
Καλαντζή, Ιωάννα
|
Abstract |
Fish and seafood are an integral part of the human diet. It is recommended as a food source due
to its multiple benefits, part of which is the intake of fatty acids that contribute to a healthy
cardiovascular system. However, the presence of high concentrations of some metals and
metalloids can negatively affect the lipid content of fish. Specifically, the presence of lipid
compounds containing an arsenic atom (75As) in their molecule have been observed in various
fish species. These compounds are referred to as arsenolipids. Their recent discovery and the
absence of extensive research on the toxicity of these species creates the need for their further
investigation.
In this work, a chromatographic method (reversed-phase liquid chromatography – RPLC)
coupled with Orbitrap mass spectrometry (ESI-Orbitrap-MS) is applied for the determination
of arsenolipids of the two main categories that have been detected in fish samples, hydrocarbons
and fatty acids containing arsenic (AsHCs and AsFAs, respectively). The method is applied to
four different fish species from the Aegean Sea (red mullet, common pandora, European hake,
and anchovy) caught from the Straits of Artemisium and the exit of the Pagasitic Gulf.
Arsenolipids of the two main categories, AsHCs and AsFAs, are detected in all samples, at
different concentrations for each fish species and in a range of total concentrations expressed
as arsenic from 18.1±1.1 ppb to 84.0±25.1 ppb.
Apart from the determination of arsenolipids, the aim is to examine the level of differentiation
of the presence of arsenolipids on three basic axes. Firstly, the effect of gender (red mullet,
common pandora) is examined, secondly the effect of the tissue (muscle, stomach content,
gonads, and gills of hake) and lastly the effect of the fish species. Differentiations are observed
in terms of gender, which are mainly due to fat content and different metabolic processes
resulting from hormonal differences between the two genders during the oviparity period.
Differentiations are also observed between tissues, which is probably due to metabolic
processes, fat content and tissue composition, as well as due to the different blood supply of
each tissue. Finally, differentiations are observed in arsenolipid content between species, which
is partly due to environmental and dietary factors and partly to fat content and metabolic
processes.
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Language |
Greek |
Subject |
Anchovy - Engraulis encrasicolus |
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Arsenic |
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Arsenolipids |
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Common Pandora - Pagellus erythrinus |
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European hake - Merluccius merluccius |
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High Resolution Mass Spectrometry |
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Inductively Coupled Plasma Mass Spectrometry |
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Red mullet - Mullus barbatus |
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Reversed Phase Chromatography |
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Αρσενικό |
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Αρσενολιπίδια |
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Γαύρος |
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Κουτσομούρα |
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Λυθρίνι |
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Μπακαλιάρος |
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Φασματομετρία Μάζας Επαγωγικά Συζευγμένου Πλάσματος |
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Φασματομετρία Μάζας Υψηλής Διακριτικής Ικανότητας |
|
Χρωματογραφία Αντίστροφης Φάσης |
Issue date |
2024-07-19 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
|
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/4/c/e/metadata-dlib-1720780222-277512-32383.tkl
|
Views |
15 |