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Identifier

000457045

Title

Προσδιορισμός ενώσεων αρσενικού με την τεχνική HPLC- ICP-MS σε βενθικά και πελαγικά ψάρια συλλεγμένα από τα στενά του Αρτεμισίου

Alternative Title

Arsenic speciation analysis using HPLC-ICP-MS in benthic and pelagic fish collected from the Artemision Straits

Author

Περγάμαλη, Αναστασία Δ.

Thesis advisor

Περγαντής, Σπυρίδων

Abstract

The main exposure to arsenic (As) of the human population, especially Greeks, is due to seafood and mainly fish and crustaceans. The Greek diet is largely based on seafood, which contains elevated levels of (As) in various chemical forms. Therefore, As speciation is important in order to determine the different As species and the amounts ingested by humans in order to make more reliable and accurate estimates of the risks posed by the specific species. In the present work, an ion exchange chromatography method coupled with inductively coupled plasma mass spectrometry (ICP-MS) is developed for the analysis of watersoluble As species in fish samples collected from the Straits of Artemisium. The specimens collected were Mullus barbatus, Pagellus Erithrinus, Merluccius Merluccius and Engraulis encrasicolus. Arsenobetaine (AsB) is the major As species in all tissues ranging from (6,1-85,6 mg As/kg) followed by tetramethyl arsenic (TMA) at lower concentrations ranging from (0,032-2,57 mg As/kg) which was also detected in all tissues. Dimethylarsenic acid (DMA) was detected in all species to be analyzed except for Pagellus Erithrinus, inorganic arsenic As(+3) only in the stomach contents of Merluccius Merluccius, and inorganic arsenic As(+5) in Engraulis encrasicolus and in the stomach contents of Merluccius Merluccius. In addition, in the present study, through statistical analysis, conclusions are drawn on the effect of sex (Mullus barbatus, Pagellus Erithrinus), tissue (Merluccius Merluccius: muscle, gonads, gills, liver, stomach contents) and type of fish (benthic, pelagic) on the speciation of As. Based on the information concerning the statistical analysis and the help of the bibliographic references, it appears that the differences in the concentrations in Mullus barbatus and Pagellus Erithrinus are due to the different sizes and diet by sex, the tissues of Merluccius Merluccius in the diet and the bioaccumulation capacity of each tissue and to the different fish species in their eating habits, size and way of collecting food.

Language

Greek, English

Subject

As(+3)

As(+5)

AsB

DMA

TMA

Issue date

2023-07-27

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses