Your browser does not support JavaScript!

Post-graduate theses

Current Record: 562 of 642

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000035758
Title Σύνθεση των 5'-tert-Βουτυλοκετονών της ριβο - και 2'-Δεοξυριβο-Γουανοσίνης για την ανεξάρτητη παραγωγή C-5' Νουκλεοζιτικών ριζών
Author Γεωργανάκης, Δημήτριος
Thesis advisor Γκιμήσης, Αθανάσιος
Abstract One of the most common actions of oxidative damage in the DNA is the abstraction of a hydrogen atom from the nucleotide deoxyribose. The chemospecific synthesis of nucleoside models as precursors for the formation of free radicals on the deoxyribose positions, has helped the study of the oxidative damage reaction pathways and the understanding of the mechanisms that take place. The C-5’ chemistry has an important part in oxidative damage pathways due to its favorable position for abstraction in the minor groove of the DNA double helix. The formation of a free radical on the C-5’ position has been the goal of the present study. In order to achieve a chemospecific formation of a C-5’ radical we placed a photolabile group like tert-butyl ketone on the 5’ position of guanosine and 2’-deoxyguanosine substrates. The photolabile groups underwent a photolysis reaction under ultraviolet radiation that led to the formation of the C-5’ radical and some useful results that can help the understanding of the mechanisms that takes place after the formation of the radical. The photolysis of the photolabile precursors under anaerobic conditions and the presence of reductive compounds leads to the formation of two new products as a result of two different and competitive reaction pathways, the reduction pathway (A) and the cyclisation pathway (B). The 5’-8 cyclopurine has also been identified after the radiation of DNA solutions, and is considered to be a poor substrate for the repair enzymes of DNA and a potential cytotoxic compound. The formation of this product under an intramolecular attack of the C-5’ radical to the purine has offered
Language Greek
Issue date 2002-07-01
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/e/a/a/metadata-dlib-2002georganakis.tkl Bookmark and Share
Views 180

Digital Documents
No preview available

Download document
View document
Views : 1