Doctoral theses
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Identifier |
000424383 |
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h |
Title |
A point-of-care, microfluidic-based microscanner for the identification and counting of single cells |
Alternative Title |
Σύστημα μικρού όγκου ,οπτικής σάρωσης μικρορευστονικού καναλιού για την ταυτοποίηση και ανίχνευση μεμονωμένων κυττάρων στο σημείο περίθαλψης |
Author
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Κορομπίλη, Γεωργία
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Thesis advisor
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Χρόνης, Νικόλαος
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Reviewer
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Χατζηνικολαϊδου, Μαρία
Αλεξόπουλος, Λεωνίδας
Τζωρτζάκης, Στέλιος
Κιοσέογλου, Γεώργιος
Μισιάκος, Κωνσταντίνος
Γκιζελή, Ηλέκτρα
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Abstract |
Blood cell counting is related to the diagnosis and therapy of various diseases – infections, blood disorders, cancer treatment. However, the time consuming procedures and the increased cost of standard medical equipment act as serious obstacles in the early screening of a disease. To meet this challenge, there is an emerging trend towards a patient-centered healthcare that provides medical examination and diagnosis at the point of care. Profiting from recent advances in Micro-ΕlectroΜechanical systems, micro-optics and micro-fluidics, the present thesis attempts to present a platform technology for blood cell identification and counting at the point of care. The developed microscanner consists of 3 novel components: (1) a microlens array enabling extended sample imaging with single cell resolution in trans-illumination imaging scheme (2) a novel design, integrating a micro-LED array and a mini-lens array on the same silicon chip for epi-illumination sample imaging and (3) a microfluidic chip for specific cell capturing from undiluted, whole blood samples. The characterization process of the individual subsystems demonstrates: (a) high optical resolution (1μm) under conditions of maximum magnification in trans-illumination scheme; (b) the ability to screen a large sample surface (2.5 cm2); (c) the achievement of 8.8μm resolution in reflection based scheme; (d) the reduction of the structure’s optical length below 2.5 mm and (e) the achievement of a clinically negligible error in white blood cell capturing in the microfluidic device, below 3.8%, compared with flow cytometry. The developed system constitutes a platform technology; it is applicable to various diseases. The conducted research proves the feasibility of single cell identification and counting in full compliance with the requirements of World Health Organization for point-of-care devices: they need to be affordable, easy-to-use, battery operated and deliverable unconditionally to all.
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Language |
English |
Subject |
Cell fractionation |
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Micro-led on silicon |
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Microfluidic design |
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Microsystem design |
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Mini-lens array |
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Miniaturized microscopes |
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Reflection -based imaging |
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Απεικόνιση ανακλώμενου φωτός |
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Απομόμωση κυτταρικών πληθυσμών |
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Μικρορευστονικές διατάξεις |
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Μικροσυστήματα μικροσκοπίας |
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Μικρό-led σε πυρίτιο |
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Συστοιχία μικρό-φακών |
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Σχεδιασμός μικροσυστημάτων |
Issue date |
2019-11-29 |
Collection
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School/Department--School of Sciences and Engineering--Department of Materials Science and Technology--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/d/2/9/metadata-dlib-1572610107-230194-31467.tkl
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Views |
588 |