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| Identifier |
000382154 |
| Title |
Non-linear micro/nanolithography with short-pulse lasers : applications on biomaterials and biosensors |
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Author
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Τερζάκη, Κωνσταντίνα
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Thesis advisor
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Μητράκη, Άννα
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Reviewer
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Βαμβακάκη, Μαρία
Φωτάκης, Κωνσταντίνος
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| Abstract |
The present PhD thesis focuses on the combination of two strategies: the top-down
laser fabrication (i.e. the possibility to control the scaffold geometry and micro-nanotopography)
and the bottom-up design possibilities of the self-assembling peptides
(driving self-assembly from the nanoscale to millimeter scale) for the development of
biocompatible high-precision scaffolds with complex architectures. The positioning
and integration of self-assembled peptides into devices fabricated with femtosecond
laser technologies was examined for applications in the field of biomaterials and
photonics.
3D scaffolds with microscale features were fabricated with Direct femtosecond Laser
Writing (DLW). The 3D structures were synthesized using a novel zirconiumcontaining
organic-inorganic photosensitive material incorporating a metal-binding
organic monomer in order to be further functionalized with metal-binding peptide
fibrils. The scaffolds’ resolution in structurability and metallization quality was
investigated as well as their mechanical properties and their biocompatibility.
The precise positioning of self-assembled peptide fibrils, their controlled assembly
and their integration into microsystems was investigated. Conductive 3D metallic
micro/nanostructures were fabricated using a silver plating technique and
subsequently previously studied metal-binding peptide fibrils were selectively
positioned over the metalized structures forming oriented peptide bridges which were
metalized in situ. The developed system can be proposed for conductivity
measurements of self-assembled peptide fibrils and its use in the field of biosensing is
highly envisaged.
Based on a previously studied octapeptide building block, bi-functional selfassembling
oligopeptides were designed, having acidic amino acids in their sequence
in order to nucleate calcium phosphate as well as metal ions. The self-assembled
peptide fibrils were positioned on gold-ion functionalized 3D structures and
subsequently acted as secondary scaffolds for the deposition of calcium phosphates
aiming at hard tissue regeneration. Pre-osteoblastic cell adhesion, proliferation and a
statistically significant increase of biomineralization makes the scaffold a promising
method for bone tissue engineering.
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| Language |
English |
| Issue date |
2014-01-22 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Materials Science and Technology--Doctoral theses
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Type of Work--Doctoral theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/1/4/7/metadata-dlib-1390648706-777817-29804.tkl
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| Views |
311 |
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