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Identifier

000453846

Title

Συγκριτική μελέτη μοριακών τεχνικών και ανοσοφθορισμού στην ανίχνευση κυκλοφορούντων καρκινικών κυττάρων στο αίμα ασθενών με καρκίνο του μαστού

Alternative Title

A comparison of three methods for the detection of circulating tumor cells in patients with early and metastatic breast cancer

Author

Πολιτάκη, Ελένη

Thesis advisor

Μαυρουδής, Δημήτριος

Reviewer

Γεωργούλιας, Βασίλειος
Λιανίδου, Εύη
Αγγελάκη, Σοφία
Θεοδωρόπουλος, Παναγιώτης
Τζαρδή, Μαρία
Στειακάκη, Ευτυχία

Abstract

Background: Several techniques have been developed for the detection of circulating tumor cells (CTCs) in patients with breast cancer. We designed this study to compare the CellSearchTM system, with real time reverse transcription quantitative polymerase chain reaction (RT-qPCR) and an immunofluorescent method for CTC detection in patients with breast cancer. Methods: Blood was obtained from patients with early and metastatic breast cancer before the initiation of adjuvant or first-line chemotherapy. Different aliquots of the same blood sample were evaluated for the presence of CTCs by the CellSearchTM System, the RT-qPCR and by double immunofluorescence (IF) microscopy. Results: In early breast cancer, using the CellSearchTM System, 37% and 16.5% of patients were CTC-positive (cut-offs ≥1 and ≥2 CTCs per 23 ml of blood), 18.0% by RT-qPCR and 16.9% by IF. In the metastatic setting, 34.8% of patients had ≥ 5 and 53.7% had ≥ 2 CTCs per 7.5 ml of blood, 37.8% were CTC-positive by RT-qPCR, and 28.5% by IF. In 60.8% of cases presenting discordant results by CellSearchTM and RT-qPCR, the differential expression of EpCAM (Epithelial cell adhesion molecule) and CK19 on CTCs, could explain the discrepancies observed. Conclusion: A significant concordance in CTC detection between the CellSearchTM and RT-qPCR was observed in metastatic setting. CTC detection by either method had prognostic relevance in metastatic patients. Discordant results could be explained in part by CTC heterogeneity.

Language

Greek

Subject

Cell search system

Immunofluorescence

Issue date

2023-04-05

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/7/8/2/metadata-dlib-1676368064-858978-5548.tkl