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Title Βιοαποικοδόμηση απόβλητων τυροκομείου από το βακτήριο Pseudomonas sp. phDV1
Alternative Title Biodegradation of dairy waste by the bacteria Pseudomonas sp. phDV1
Author Παππά, Ευγενία
Thesis advisor Τσιώτης, Γεώργιος
Abstract Plastics are today a critical problem for human society, as they are characterized as particularly harmful to the natural environment in the long term and to date, no easy way to achieve their management has been found. One way that appears to be more effective than recycling and discarding is to gradually replace petroleum-based plastics with bioplastics, which are much more environmentally friendly due to their ability not to be synthesized from microorganisms, as well as due to their significant biodegradability. One such type of biopolymers are polyhydroxyalkanoates (PHAs), which carry a range of desirable characteristics that make them ideal alternatives for this role. Polyhydroxybutyric acid (PHB) is the simplest and most widely studied PHA to date. The bacterium Pseudomonas sp. phDV1 is a Gram-negative bacterium known to be able to utilize waste that is toxic to both nature and humans for its growth. Cheese whey is also an important polluting waste, which is rich in organic components, but its disposal in nature is prohibited. At the same time, it is known that the specific microorganism can produce PHB when under stress conditions. The PHBs in this specific bacterium are produced intracellularly and are organized into granules, known as carbosomes. These structures display on their surface proteins responsible for the structure (phasins), regulation (regulatory factors, PhaR), production (synthases, PhaC) and degradation (depolymerases, PhaZ) of PHBs. The present work focuses on the utilization of cheese whey as a basic substrate for the growth of the wild type of Pseudomonas sp. phDV1 in different conditions each time, as well as detecting PHB in each different condition studied. Furthermore, it is being studied whether the growth of this bacterium in the specific waste, can reduce the total pollutant load of the cheese whey, making it safer for the environment.
Language Greek
Subject Bioplastics
Bιοπλαστικά
Cheese whey
PHB
Tυρόγαλα
Issue date 2024-07-19
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/0/8/4/metadata-dlib-1720434835-17125-31960.tkl Bookmark and Share
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