Graduate theses
Current Record: 4 of 1663
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Title |
Μελέτη του ΑΤΡ ως υπόστρωμα της 15-Λιποξυγενάσης-1 |
Alternative Title |
Study of ATP as a substrate of 15-Lipoxygenase-1 |
Author
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Βραχνά, Μαρία
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Thesis advisor
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Ελευθεριάδης, Νικόλαος
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Abstract |
15-Lipoxygenase-1 (15-LOX-1) is a key enzyme in mammalian cells, mainly involved in lipid
metabolism. It is located in the cytoplasm of immune and epithelial cells and targets
polyunsaturated fatty acids such as arachidonic and linoleic acids. By catalyzing the oxygenation of
fatty acids at the 15th carbon position, it produces hydroperoxyacetate tetraenoic acid derivatives
(HPETE), which serve as precursors such as lipid bioactive mediators. The importance of 15- LOX1 extends to various physiological and pathological processes, including inflammation,
atherosclerosis, cancer and neurodegenerative diseases. Overall, 15-lipoxygenase-1 is characterized
as a multifaceted, important enzyme for lipid metabolism and cellular physiology, thus making it a
prime target for therapeutic interventions in different diseases. In this thesis, the heterologous
expression and purification process of the enzyme is first presented and then the ability to hydrolyze
ATP by ADT and inorganic phosphorus is examined. After the hydrolysis of ATP by the enzyme
was established and quantified, factors likely to affect the rate of hydrolysis were examined, such as
different concentrations of ATP and enzyme, reaction incubation time and thermal incubation of the
enzyme at different temperatures. Then, the inhibition of catalytic ATP hydrolysis using two
inhibitors and the dependence of ATP hydrolysis under the presence of substrate were investigated.
Finally, the ATP hydrolysis reaction of the enzyme under the coexistence of both the enzyme
substrate and a synergistic inhibitor was studied.
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Language |
Greek |
Subject |
ATP hydrolysis |
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ATP quantification |
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Factors affecting ATP hydrolysis |
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Heterologous expression |
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Protein purification |
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Ετερόλογη έκφραση |
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Καθαρισμός πρωτεΐνης |
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Παράγοντες που επηρεάζουν την υδρόλυση του ΑΤΡ |
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Ποσοτικοποίηση ΑΤΡ |
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Υδρόλυση ΑΤΡ |
Issue date |
2024-07-19 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/9/a/b/metadata-dlib-1719900493-564719-23532.tkl
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Views |
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