Graduate theses
Current Record: 656 of 1659
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| Title |
Non-targeted arsenic speciation in peptides using ultra-high resolution Mass Spectrometry |
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Author
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Τρασανίδου, Λευκοθέα
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Thesis advisor
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Περγαντής, Σπυρίδων
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| Abstract |
The scope of this thesis was, to study the interaction of biomolecules and specifically peptides, with arsenic (As), which requires the development of analytical methods for the analysis of trivalent arsenic existing in As-glutathione (As-GSH) complexes͘ without the need for extended sample preparation, using very high resolution mass spectrometry and with low limits of detection. Method optimization was carried out by using a series of arsenic-containing peptides, used as standard compounds. More specifically, a synthetically prepared series of As-complexes, including (a-glutamyl-cysteinyl-glycinyl) dimethyl-thio-arsinite or dimethylarsinic glytathione (DMAG), di(glutamyl-cysteinyl-glycinyl)methyl- dithio-arsonite or methylarsonate glutathione (MADG), and tri(glutamyl-cysteinyl-glycinyl)trithio-arsenite or arsenite glutathione (ATG), were used. To analyze these compounds mass spectrometry with various ionization methods was developed. Electrospray (ESI-MS) and sonic spray ionization mass spectrometry (SSI-MS) as well as tandem mass spectrometry (MS/MS) were used, operated in the selected reaction monitoring (SRM mode), in order to make mass measurements and structural configuration predictions. Electrospray ionization -Fourier transform-ion cyclotron resonance mass spectrometry (ESI- FT-ICR-MS) and also matrix-assisted laser desorption/ionization (MALDI) FT-ICR-MS were used to make accurate mass measurements. Analyte samples were analyzed directly from solution, and also eluting from an HPLC column (Hypersil C18), prior to their detection using their accurate m/z values. The three As-GSH compounds were analyzed using HPLC -ESI-FT-ICR-MS both in aqueous solutions and after spiking into biological extracts, specifically in the seaweed (hijiki) extracts.
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| Language |
Greek |
| Issue date |
2016-03-18 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/d/5/0/metadata-dlib-1455088311-578815-10350.tkl
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| Views |
205 |
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