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Identifier

000466079

Title

Προσδιορισμός αρσενολιπιδίων σε τέσσερα είδη ψαριών του Αιγαίου

Alternative Title

Determination of arsenolipids in four fish species of the Aegean sea

Author

Δρακωνάκη, Χρυσοβαλάντω - Μαρία Ν.

Thesis advisor

Περγαντής, Σπυρίδων

Reviewer

Σπύρος, Απόστολος
Καλαντζή, Ιωάννα

Abstract

Fish and seafood are an integral part of the human diet. It is recommended as a food source due to its multiple benefits, part of which is the intake of fatty acids that contribute to a healthy cardiovascular system. However, the presence of high concentrations of some metals and metalloids can negatively affect the lipid content of fish. Specifically, the presence of lipid compounds containing an arsenic atom (⁷⁵As) in their molecule have been observed in various fish species. These compounds are referred to as arsenolipids. Their recent discovery and the absence of extensive research on the toxicity of these species creates the need for their further investigation. In this work, a chromatographic method (reversed-phase liquid chromatography – RPLC) coupled with Orbitrap mass spectrometry (ESI-Orbitrap-MS) is applied for the determination of arsenolipids of the two main categories that have been detected in fish samples, hydrocarbons and fatty acids containing arsenic (AsHCs and AsFAs, respectively). The method is applied to four different fish species from the Aegean Sea (red mullet, common pandora, European hake, and anchovy) caught from the Straits of Artemisium and the exit of the Pagasitic Gulf. Arsenolipids of the two main categories, AsHCs and AsFAs, are detected in all samples, at different concentrations for each fish species and in a range of total concentrations expressed as arsenic from 18.1±1.1 ppb to 84.0±25.1 ppb. Apart from the determination of arsenolipids, the aim is to examine the level of differentiation of the presence of arsenolipids on three basic axes. Firstly, the effect of gender (red mullet, common pandora) is examined, secondly the effect of the tissue (muscle, stomach content, gonads, and gills of hake) and lastly the effect of the fish species. Differentiations are observed in terms of gender, which are mainly due to fat content and different metabolic processes resulting from hormonal differences between the two genders during the oviparity period. Differentiations are also observed between tissues, which is probably due to metabolic processes, fat content and tissue composition, as well as due to the different blood supply of each tissue. Finally, differentiations are observed in arsenolipid content between species, which is partly due to environmental and dietary factors and partly to fat content and metabolic processes.

Language

Greek

Subject

Anchovy - Engraulis encrasicolus

Arsenic

Arsenolipids

Common Pandora - Pagellus erythrinus

European hake - Merluccius merluccius

High Resolution Mass Spectrometry

Inductively Coupled Plasma Mass Spectrometry

Red mullet - Mullus barbatus

Reversed Phase Chromatography