Post-graduate theses
Current Record: 4983 of 6695
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Identifier |
000300743 |
Title |
Ανάπτυξη μεθόδων υγρής χρωματογραφίας με φασματομετρία μαζών για ανίχνευση και χαρακτηρισμό νέων μορφών αρσενικού (θειοαρσενοσάκχαρα) σε βιολογικά δείγματα |
Alternative Title |
Develpoment of liquid chromatography mass spectrometry methods for detection and characterization of new arsenic species (thioarsenosugars) in bilogical samples |
Author
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Κανάκη, Αικατερίνη Χαρίτωνος
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Thesis advisor
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Περγαντής, Σπυρίδων
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Abstract |
Arsenic is ubiquitous in the environment, with its toxicity being
dependant on its oxidation state and its chemical form. Humans are exposed to
arsenic, mainly through their food and drinking water, while quite high
concentrations of this element are found in seafood. Algae and organisms
feeding on algae are rich in oxoarsenosugars (arsenic containing ribofuranosides,
((CH3)2RAs=O, R = ribose moiety). The toxicity of these compounds, which are
considered to be metabolized in vivo to other species of unknown toxicity, has
not been fully evaluated yet.
Following recent reports on the detection of a new group of
thioarsenosugars (((CH3)2RAs=S) in marine molluscs, we explored in the present
study the presence of additional species of this group in various biological
samples. For this reason High Performance Liquid Chromatography (HPLC)
coupled with Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and
Electrospray Tandem Mass Spectrometry (ES-MS/MS) methods were
developed. A series of thioarsenosugar standards was prepared, through the
reaction of the corresponding oxoarsenosugars with H2S.
First, conventional anion exchange chromatographic conditions were
modified to achieve efficient elution of the thioarsenosugars. This required
relatively high contents of methanol in the mobile phase (up to 40%). However,
the high amount of methanol used with this method was not suitable for direct
hyphenation with ICP-MS, without a specially adapted interface. Therefore,
reversed phase anion pairing HPLC with low methanol content (5%) in the mobile
phase was developed and used. The combined application of both analytical
techniques revealed the presence of two novel thioarsenosugars in marine
bivalves and marine algae. Moreover, this is the first time that the detection of
thioarsenosugars in marine algae is reported.
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Language |
Greek |
Subject |
Arsenic speciation |
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Electrospray |
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Inductively Coupled Plasma |
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Mass Spectrometry |
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Tthioarsenosugars |
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Ειδοταυτοποίηση αρσενικού |
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Επαγωγικά Συζευγμένο Πλάσμα |
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Ηλεκτροψεκασμός |
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Θειοαρσενοσάκχαρα |
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Φασματομετρία |
Issue date |
2007-05-18 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/f/1/9/metadata-dlib-3c7b70cb951648a0325672700b928559_1268124984.tkl
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Views |
213 |