Post-graduate theses
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Identifier |
000444086 |
Title |
In vitro system of an atherosclerosis model |
Alternative Title |
In vitro σύστημα ενός μοντέλου αθηροσκλήρωσης |
Author
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Λάσκου, Μαρία
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Thesis advisor
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Fisher, Edward A.
Kardassis, Dimitris
Tsatsanis, Christos
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Abstract |
Vascular smooth muscle cells (VSMCs) and endothelial cells (ECs) are two major cell types involved in
arterial wall development, blood vessel integrity and homeostasis. In addition, they play a key role in
atherosclerotic plaque formation. Atherosclerosis is a chronic cardiovascular inflammatory disease
attributed to high blood cholesterol, obesity and/or diabetes, and it involves a plethora of different cell
types, including VSMCs and ECs. These cells undergo phenotypical changes which further promote
atherosclerosis. During disease progression VSMCs tend to differentiate from a contractile state to
macrophage-, osteochondrocyte- or myofibobrast-like cells. ECs also transdifferentiate to mesenchymallike cells, such as myofibroblasts or smooth muscle cells, through the Endothelial to Mesenchymal
transition (EMT). Understanding VSMC-EC function, cross-talk and phenotypic transdifferentiations
during atherosclerosis may offer insights for prevention and treatment of atherosclerotic cardiovascular
disease (CVD). Many in vitro systems have been focused on exploring these interactions and cross-talk;
however, to our knowledge, there is not any single system that can truly recapitulate the full potential
of cell phenotypical modulation in an atherosclerosis background. Here, we introduce a novel co-culture
of human VSMCs and ECs after cholesterol treatment. Our system presents similar phenotypical changes
of VSMCs, such as downregulation of contractile markers and upregulation of macrophage-like markers.
Developing an in vitro system as an atherosclerosis model can be beneficial in studying cellular function,
transdifferentiation and their underlying molecular mechanisms, as well as drug treatments for CVD.
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Language |
English |
Issue date |
2021-12-01 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/f/4/metadata-dlib-1639653007-795867-25913.tkl
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Views |
240 |
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