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Identifier

uch.biology.msc//2005skreka

Title

Επιβεβαίωση των υποψηφίων γονιδίων-στόχων των mir15a και mir16

Author

Σκρέκα, Κωνσταντινιά

Abstract

Micro-RNAs are ribonoucleotides of 21-24 bp, which cause gene silencing called PTGS (post transcriptional gene silencing) in plants and RNAi (RNA interference) in animals. Their binding sites, to which they match imperfectly, are located in the 3UTR of animal genes. In the past few years micro-RNAs have been implicated in some cases of cancer, while oncogenes, like RAS, have been found to be regulated by them. In the majority of Chronic Lymphocytic Leukemia (CLL) cases caused by a deletion in the chromosomal region 13q14, there is a reduction in the quantity of mir15 and mir16. The research for mir15 and mir16 target-genes carried out in our lab led to the identification of putative candidate genes. While elaborating this Master thesis, the candidate genes were analyzed using the microInspector program both to reveal whether they constitute in silico targets of mir15 and mir16 and in order to find the mir15 and mir16 binding sites in their 3UTRs. The binding sites were cloned in a luciferase expression vector and transfections were carried out. The results point out to at least one gene, the oncogene Bcl2, as being a possible mir16 target, which was recently published by Cimmino et al 2005. The characterization of mir15 and mir16 target genes, like Bcl2, is highly significant in order to resolve the reasons causing CLL.

Language

Greek

Issue date

2005-11-28

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/f/7/0/metadata-dlib-2005skreka.tkl

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