Doctoral theses
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Identifier |
000465762 |
Title |
The role of the plant cell border in cell fate |
Alternative Title |
Ο ρόλος του ορίου των φυτικών κυττάρων στην κυτταρική μοίρα |
Author
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Μεντζελοπούλου, Ανδριανή Σ
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Thesis advisor
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Μόσχου, Παναγιώτης
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Reviewer
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Σαρρής, Παναγιώτης
Παυλόπουλος, Αναστάσιος
Καλαντίδης, Κρίτωνας
Βιδάκη, Μαρίνα
Φιλιππίδη, Εμμανουέλα
Σπηλιανάκης, Χαράλαμπος
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Abstract |
Molecular condensation is crucial for polarity establishment and stress responses in
biological systems. However, the role of condensation in plants is still fragmentary. In the
first part of this PhD thesis, I will refer to a cytoplasmic condensate, like the condensate of
processing bodies (PBs). I will describe PBs by deciphering at first their protein
composition, using the PB core component DECAPPIN PROTEIN 1 (DCP1) as a bait. It is
known that PBs reside in the cytoplasm. However, protein datasets revealed a big network
of proteins related to the plasma membrane and to the trafficking of vesicles. Thus, I will
approach how these condensates can become membrane–bound or be associated with
membrane compartments. Additionally, I found that PBs properties can be regulated by the
actin polymerization cAMP receptor (SCAR)–WASP family verprolin homologous (WAVE)
complex, as another network of proteins in PBs is related to actin remodelling. The
SCAR/WAVE complex interacts with DCP1 at cell edges/vertices of the Arabidopsis root
and facilitates the actin nucleation process and directional growth. In the second chapter,
I will approach the RNA composition of PBs, defining networks related also to
hormonal/wounding responses. The SCAR/WAVE complex enables the RNA release from
PBs towards the translation machinery modulating hormonal (ethylene) signalling and plant
responses to wounding and regeneration. More specifically, RNAs and their cognate
proteins are enriched in PBs and comprise of common networks, named “co-regulons”.
These co-regulons are processed in PBs through the interplay between decay and storage
and RNAs are released to translation upon stress/wounding to facilitate plant responses.
In the third chapter, I will try to delineate how a condensate can affect plasma membrane
properties. I will refer to the lipid–transferase and condensate Sec14–like nodulin (SEC–
FOURTEEN HOMOLOGUE 8) SFH8, which undergoes a liquid–to–solid state transition at
the plasma membrane to maintain the polarity of auxin efflux carriers. I will describe the
SFH8 interaction with membranes, through its interaction with the specific lipid
phosphatidylserine (PS). SFH8 is linked to PS, as removal of SFH8 or vice versa results
in compromised PS clustering on the PM. Thus, this PS-SFH8 link can provide an example
of how condensates can affect plasma membrane properties and, thus, root development.
Finaly, I will try to describe a mechanosensing probe, which can be used to detect changes
in plasma membrane properties. This mechanical–stretch activated sensor deriving from
the human mechanosensing channel Piezo1 and expressed in Arabidopsis plants, may be
a useful tool for the detection of plasma membrane stretching alterations.
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Language |
English |
Subject |
Membrane stretching |
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Plant condensates |
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Plasma membrane |
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Polarity |
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Processing bodies |
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RNA biology |
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Wounding |
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Βιολογία RNA |
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Μηχανική τάση |
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Πλασματική μεμβράνη |
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Πολικότητα |
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Συμπυκνώματα φυτών |
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Τραυματισμός |
Issue date |
2024-07-26 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/3/a/d/metadata-dlib-1719497469-495150-12306.tkl
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Views |
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