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Identifier

000465744

Title

Development of a multiparametric label-free imaging system for the early diagnosis of neurodegenerative disorders through the ocular cavity

Alternative Title

Ανάπτυξη συστήματος πολυπαραμετρικής απεικόνησης εγγενούς αντίθεσης για την πρώιμη διάγνωση νευροεκφυλιστικών αλλοιώσεων μέσω του οφθαλμού

Author

Μαυράκης, Κωνσταντίνος

Thesis advisor

Ζαχαράκης, Ιωάννης

Reviewer

Παπάζογλου, Δημήτιος
Τζωρτζάκης, Στυλιανός
Τσιλιμπάρης, Μιλτιάδης
Τζεράνης, Δημήτριος
Γκουρίδης, Γεώργιος
Βασιλάκης, Γεώργιος

Abstract

In this novel imaging system, we have combined three non-invasive and label free imaging tech- niques: Optoacoustic microscopy (OAM) , non-linear microscopy (NLM) and Stimulated Raman Scattering (SRS) microscopy . OAM exhibits resolution comparable to that of optical microscopy, but can penetrate deeper into high-scattering tissue. The increased possibilities stem from the fundamental scattering difference between light and sound, which constitute the signal for optical microscopy and OAM, respectively. NLM includes two photon excitation fluorescence (TPEF) and Second Harmonic Generation (SHG) . OAM, TPEF, SHG and SRS are combined complementary and thus reveal a wide range of discrete and non-overlapping information. TPEF presents crisp contrast between bio-molecules that possess different excitation or emission spectra. SHG can provide information about biological structures such as lipid depositions while OAM pinpoints photon absorbing molecules with non-radiative relaxation. SRS imaging provides chemical information with high specificity allowing molecular pattern recognition inside tissues and their association with diseases and pathological conditions. Using these techniques, even sensitive and unreachable structures like the retina and the ocular cavity can be investigated with high resolution. The nerve fibers in the ocular cavity are essentially an extension of the central nervous system and since several neurodegenerative disorders present symptoms in the cavity, they could be diagnosed before the manifestation of conventional symptoms. The presence of possible bio-markers was investigated with TPEF, SHG and OAM in retina samples from mouse models of the Alzheimer’s disease.

Language

English

Subject

Alzheimer's disease

Microscopy

Opto-acoustics

Retina

Two photon fluorescence

Αλτσχάιμερ

Αμφιβληστροειδής

Διφωτονικός φθορισμός

Μικροσκοπία