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Identifier |
000363516 |
Title |
In vivo ανάλυση της φυτικής 3'-5' εξωνουκλεάσης ERL1 |
Alternative Title |
In vivo analyses of plant 3'-5' exonuclease ERL1 |
Author
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Βαμβακά, Ευγενία
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Thesis advisor
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Καλαντίδης, Κρίτων
Τσαγρή, Ευθυμία
Κοτζαμπάσης, Κωνσταντίνος
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Abstract |
Chloroplasts are subcellular organelles combine features from prokaryotic
organisms, because of their origin from symbiotic photosynthetic bacteria, and also
from eukaryotes, due to their adaptation in such environment. In plants chloroplasts
possess their own genome, which during endosymbiosis, lost its genetic autonomy
and many genes transferred to the nucleus. Nevertheless some genes remained at
chloroplasts and these genes encode components of photosynthetic and
transcription-translation apparatus of the plastids.
The control of gene expression in plastids is a post-transcriptional process with RNA
splicing, turnover, editing and processing being the primarily procedures. RNA
processing includes cleavage of polycistronic transcripts and also the maturation of
5’ and 3’ ends of them. Endonucleolytic and exonucleolytic mechanisms participate
in mature ends formation of transcripts. For mature 3’ ends responsible are 3’-5’
exonucleases, such as PNPase and RNase R1, which are parts of ribosomal RNA
metabolism.
This category includes also DEDD exonucleases, part of them are the ERI1s, which
use as substrates double stranded DNA /RNA molecules and catalyze their single
stranded 3’ ends. ERI1s participate in pathways of RNA silencing and processing of
rRNAs in many organisms. In plants it seems that ERL1 do not play any role in RNAi,
but participate in rRNA processing, 5S specifically.
The initial query of this thesis was to clone ERL1 from N.benthamiana and A.thaliana
in order to overexpress and downregulate it. The plasmid was checked for their
functionality. Afterwards specific chloroplastic genes have been checked for
differences in expression levels. Simultaneously we produced transgenic plants
N.benthamiana with ERL1 Hairpin and try to establish their functionality. The aim
was the use of them in chloroplastic genes analyses. Finally, transgenic plants
A.thaliana were grown and analyzed for the study of the same genes and the
comparison of them between these two plant species.
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Language |
Greek |
Subject |
Chloroplast metabolish |
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DEDDh family |
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Post-transcriptional processes |
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RNA processing |
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RNA επεξεργασία |
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Επεξεργασία ριβοσωμικού RNA |
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Οικογένεια DEDDh |
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Χλωροπλαστικός μεταβολισμός |
Issue date |
2011-03-18 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/e/5/7/metadata-dlib-345f2fa23b1d9b8217ef3a4485ab1c74_1296462100.tkl
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Views |
272 |