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Identifier |
000417852 |
Title |
Μελέτη των μεταβολών της έκφρασης γονιδίων που σχετίζονται με το μεταβολισμό και τη δράση χημειοθεραπευτικών στον πρωτοπαθή όγκο και στους επιχώριους και μεσοθωρακικούς διηθημένους λεμφαδένες σε χειρουργήσιμο μη μικροκυτταρικό καρκίνωμα του πνεύμονα (ΜΜΚΚΠ) |
Alternative Title |
Association of BRCA1, ERCC1, RAP80, PKM2, RRM1, RRM2, TS, TSP1 and TXR1 m RNA expression levels between primary tumors and infiltrated regional lymph nodes in patients with resectable non‐ small cell lung cancer |
Author
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Τρυφωνίδης, Κωνσταντίνος
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Thesis advisor
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Σουγκλάκος, Ιωάννης
Γεωργούλιας, Βασίλειος
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Reviewer
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Σταθόπουλος, Ε.
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Abstract |
Differences in gene expression levels between the primary tumors (PTs) and matched
regional lymph nodal metastases (LNs) in patients with totally excised non‐ small cell
lung cancer (NSCLC) were explored. Microdissected formalin‐ fixed paraffin‐ embedded
(FFPE) samples from (PT) and their matched infiltrated LNs, from 239 patients [183 (with
matched PT and LNs samples)‐ case& 56 PT only samples‐ control cohorts ] were
analyzed for BRCA1, ERCC1, RAP80, PKM2, RRM1, RRM2, TS, TSP1 and TXR1 mRNA
expression by quantitative real‐ time polymerase‐ chain reaction (PCR). Moderately
positive correlation between the expression of each gene in the PT and the matched LNs
was observed. Concordance rates between the PT and the LNs were: BRCA1 (67.7%),
ERCC1 (68.4%), PKM2 (63.4%), RAP80 (68.8%), RRM1 (70.9%), RRM2 (69%), TS (72.9%),
TSP1 (69.8%), TXR1 (63.7%). Expression levels and their differences were correlated with
Relapse‐ Free Survival (RFS) and Overall Survival (OS). High BRCA1 PT in patients with
squamous histology was associated with increased OS (p= 0.036). High TSP1 PT levels
were shown to be the only independent prognostic factor for OS and RFS (p= 0.023 and
p=0.007). PKM2 low levels in both PT and matched LNs were associated with better OS
irrespective of the underlying histology (p= 0.031). RRM1 discordant levels between PT
and matched LNs were associated with worse OS in squamous tumors (p= 0.019)
compared to patients with both low expression in PT and LN.TXR1 high levels in both PT
and matched LNs were associated with better OS in patients with squamous tumors (p=
0.007).These findings indicate that there is different gene expression between PT and
matched LNs which may affect the outcome in early NSCLC and therefore PT’s molecular
biology should not be the sole determinant for prognostication.
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Language |
Greek |
Subject |
Adjuvant chemotherapy |
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Gene expression |
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Lymph nodes |
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NSClC |
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Primary tumor |
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Tumor heterogeneity |
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Επικουρική χημειοθεραπεία |
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Ετερογένεια |
Issue date |
2018-12-05 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/8/6/0/metadata-dlib-1544628342-16162-24205.tkl
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Views |
260 |