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Identifier |
000429199 |
Title |
Χαρακτηρισμός ενός στελέχους Chlorella που απομονώθηκε από ποταμό στην Κρήτη και πιθανές εφαρμογές στη βιομηχανία τροφίμων και διαχείριση αποβλήτων |
Alternative Title |
Characterization of a Chlorella strain isolated from a river in Crete and possible applications in food industry and waste management |
Author
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Στρατηγάκης, Ναπολέων-Χριστόφορος Μ.
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Thesis advisor
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Γανωτάκης, Δημήτριος
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Reviewer
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Κοτζαμπάσης, Κυριάκος
Παυλίδης, Ιωάννης
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Abstract |
The present study focuses on the characterization of a novel Chlorella strain isolated
from a eutrophic site at Giofyros River (Heraklion, Crete, Greece). The aim of this study was
to evaluate this microalga for applications in the bioremediation of rich in organic matter
industrial waste waters.
The first part of this thesis focuses on the genetic characterization of the strain, the
study of a broad range of properties related to cell growth rate, as well as its resistance to
common chemical inhibitors. 18S rRNA gene analysis as well as the Neighbor- joining
phylogenetic tree inferred from partial 18S rRNA gene sequence, showed that this microalga
belongs to the Chlorella genus. Photoheterotrophically grown cells achieved remarkably high
growth rates, despite the low light intensities used in the experiments; the photosynthetic rate
under variouslight conditions was assessed as well. Higher levels of photosynthetic activity
were observed for photoautotrophic cells, while photoheterotrophic cells were more tolerant
to higher light intensities. Photoautotrophic cells were found to contain higher amounts of
carbohydrates and photosynthetic pigments, while their photoheterotrophic counterparts
showed a slightly elevated lipid content. Under both growth conditions, the cells did contain
similar protein levels. Furthermore, the strain was found to be particularly resistant to the
deleterious effects of chemical inhibitors, even at the relatively high concentrations tested.
This ability was attributed to an extensive extracellular polysaccharide matrix, which seems
to play a protective role for cell homeostasis maintenance.
In the second part of this thesis, the ability of the isolated Chlorella strain to grow in
dairy waste-waters was investigated. The cells were able to acclimate to the wastewater
media; however the growth was limited due to the lack of appropriate nutrients. Thus, the
enrichment of dairy waste-waters with low amounts of nutrients allowed higher cell growth
rates. Moreover, the bioremediation of the waste-waters by the microalga was evaluated
through the removal of sugars in the media. Chlorella cells grown in media supplemented
with dairy waste were found to have biochemical content similar to the one observed with
photoautotrophic grown cells.
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Language |
Greek |
Subject |
18S rRNA gene |
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Bioremediation |
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Dairy wastewaters |
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Growth |
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Microalgae |
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Photosynthesis |
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Photosynthetic activity |
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Ανάπτυξη |
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Βιοαποκατάσταση |
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Γαλακτοκομικά απόβλητα |
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Γονίδιο 18S rRNA |
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Μικροφύκη |
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Φωτοσυνθετική ικανότητα |
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Φωτοσύνθεση |
Issue date |
2020-03-27 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/9/b/2/metadata-dlib-1585735638-696540-7617.tkl
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Views |
586 |