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Identifier

000434274

Title

Design and method development of palaeoproteomics analysis in modern and ancient human bones

Alternative Title

Σχεδιασμός και ανάπτυξη μεθοδολογίας παλαιοπρωτεϊνωματικής ανάλυσης μοντέρνων και αρχαίων ανθρώπινων οστών

Author

Τσιμινικάκη, Κωνσταντίνα Ι.

Thesis advisor

Κοκκινίδης, Μιχαήλ

Reviewer

Αϊβαλιώτης, Μιχαήλ
Περγαντής, Σπυρίδων

Abstract

Proteomics analysis based on mass spectrometry (MS) is a widely known scientific field, involving the large-scale investigation of the structure and function of a wide range of proteins and their posttranslational modifications. Mass spectrometry-based proteomics enables the study of fields of palaeoanthropology, bio-archaeology, archaeology and forensic sciences generally. It offers valuable information about ancient proteins, protein pathways and their post-translational modifications, unlike common anthropology techniques or ancient DNA analysis. Palaeoproteomics analysis has offered a variety of protocols on different kind of samples, like art items, teeth, and bones of various organisms, in order to answer evolutionary or dietary questions, for sex estimation, as well as for dating samples. The aim of this current study was to design and develop an MS-based palaeoproteomics methodology for the study of modern and ancient human samples, particularly bones. A total of 9 fractionated samples was used, 8 relative modern and one ancient sample. Sample selection and preparation, digestion, desalting and nano liquid chromatography-tandem mass spectrometry (nLC-MS/MS) analysis were contacted following an already established protocol with noteworthy modifications. This study was successful in extracting, digesting, concentrating, and identifying proteins from modern and ancient human samples, as well as identifying unique proteins. It is necessary to proceed to a further bioinformatics analysis, including other proteomes from bacteria or fungi, in order to identify possible contaminants. In addition, there is the need to extend our sample number and it is important to design a standard sampling and cleaning methodology.

Language

English

Subject

Ancient proteins

Human bones

nLC-MS/MS

Ανθρώπινα οστά

Αρχαίες πρωτεΐνες

Issue date

2020-11-27

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses