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Identifier

000433890

Title

Extraction of mouse brain extracellular matrix by decellularization for porous scaffold fabrication

Alternative Title

Απομόνωση εξωκυττάριας μήτρας εγκεφάλου ποντικού μέσω αποκυτταροποίησης για δημιουργία πορώδους ικριώματος

Author

Μαραυγάκη, Ερασμία Ιωάννα

Thesis advisor

Γραβάνης, Αχιλλέας
Χαραλαμπόπουλος, Ιωάννης
Τζεράνης, Δημήτριος

Abstract

The extracellular matrix (ECM) is a complex of proteins and proteoglycans that contributes to normal physiology of tissues. The ECM of the Central Nervous System (CNS) is important for multiple processes, including basal lamina formation, neural communication and synaptic plasticity stabilization. Alterations to ECM due to injury (e.g. spinal cord injury, stroke) or disease (e.g. Alzheimer’s disease) can have dramatic consequences. Since ECM can simulate cells, acellular biologic scaffolds are used for tissue remodeling after damage and have promising applications in CNS regenerative medicine. In this master thesis a new decellularized process was developed in order to purify decellularized brain ECM from adult mouse brain tissue. Decellularized tissue was broken in pieces and lyophilized in order to produce ECM powder, which is a raw material appropriate for scaffold fabrication. The obtained decellularized brain ECM was characterized for its bio-compability and toxicity and demonstrated neuroprotective actions when used as a medium supplement in PC12 and NSC34 cell lines in vitro. Furthermore, the obtained decellularized brain ECM was able to regulate the adhesion of primary Embryonic Cortical Neural Stem Primary Cells (NSCs) without affecting their survival or differentiation. These results suggest that the obtained decellularized brain ECM provides neuro-protective ECM components, which can be used for fabricating porous scaffolds and potentially advance NSC-based grafts for CNS regenerative medicine applications.

Language

English

Subject

Αποκυτταροποίηση

Εξωκυττάρια μήτρα

Πορώδες ικρίωμα

Issue date

2020-12-17

Collection

School/Department--School of Medicine--Department of Medicine--Post-graduate theses

Type of Work--Post-graduate theses

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