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Identifier

000426018

Title

Απομόνωση και χαρακτηρισμός δευτερογενών μεταβολιτών από το φυτό Hypericum empetrifolium

Alternative Title

Isolation and identification of components from the plant Hypericum empetrifolium

Author

Σαμιωτάκη, Χρυσούλα Ε.

Thesis advisor

Κατερινόπουλος, Χαράλαμπος

Reviewer

Περγαντής, Σπύρος
Σπύρος, Απόστολος

Abstract

The species Hypericum empetrifolium (Clusiaceae) grows mostly in Turkey and Greece. It is used in traditional medicine by the locals as a therapeutic agent for wound healing, antioxidant and against psoriasis. Our research group opted to examine its ingredients and identify its biologically active components. Aerial parts of the plant were collected during its blooming period, dried and extracted in a Soxhlet apparatus via the sequential use of increasing polarity: hexane, dichloromethane and methanol. The extracts were subjected to further extractions and column chromatography. Since the methanol extract contains the greater range of components used as therapeutic agents, the methanol extract residue was re-extracted with petroleum ether, ethyl acetate and n-butanol. Analysis of the polar extracts with NMR and ESI-MS techniques led to the identification of a number of phenolic acids, including three “chlorogenic acid” stereoisomers (5-O-caffeoyl-(-)-quinic acid, 3-O-caffeoyl-(+)-quinic acid and 5-O-caffeoyl-epi-quinic acid) and the methyl ester of 3-O-caffeoyl-(+)-quinic acid. A second group of components contained the flavonoids hyperoside, avicularin, guaiaverin, as well as shikimic acid. 5-O-caffeoyl-epi-quinic acid was isolated from the genus Hypericum for the first time, while guaiaverin and methyl ester of 3-O-(+)-caffeoyl-quinic acid were isolated for the first time from the species of Hypericum empetrifolium.

Language

Greek

Subject

Flavonoids

Phenolic acids

Secondary metabolites

Δευτερογενείς μεταβολίτες

Φαινολικά οξέα

Φλαβονοειδή

Issue date

2019-11-29

Collection