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Identifier

000433952

Title

Μελέτη πρωτεϊνικών συμπλόκων στο βακτηριακό στέλεχος Pseudomonas sp phDV1 παρουσία φαινόλης και οκρεσόλης

Alternative Title

Study of protein complexes of the Pseudomonas sp phDV1 bacterial strain in presence of phenol and o-cresol

Author

Σπανού, Ανδρονίκη Γ.

Thesis advisor

Τσιώτης, Γεώργιος

Reviewer

Γανωτάκης, Δημήτριος
Παυλίδης, Ιωάννης

Abstract

The study of proteins and protein complexes of bacterial origin is an area of growing interest in the research of transporting and metabolizing the nutrients of a bacterium. Pseudomonas sp. phDV1, is a Gram-negative bacterium selected for its ability to degrade aromatic compounds such as phenol and ortho-cresol. In the present study, the bacterium was cultured using succinic acid, phenol, and ortho-cresol as sole carbon sources, respectively, for the purpose of proteomic analysis of water-permeable and membrane proteins / complexes of the bacterium. In order to achieve the growth of the bacterium, guided by the toxicity of the above aromatic compounds, culture attempts were made until the growth of the bacterium in the respective pollutant was achieved. The biological samples were collected from which the cytoplasmic and membrane fraction were isolated. This is followed by analysis of the protein fractions with Blue Native PAGE (1D and / or 2D electrophoresis). The identification of the separated proteins was achieved by mass spectrometry and more specifically by using Matrix Assisted Laser Desorption Ionization (MALDI -TOF MS / MS) and nano-LC-Electron Spray (ESI) MS / MS. The results obtained from the proteomic analysis are comparative studies of proteins and protein complexes that are identified according to each condition. The present work is a new approach to the study of Pseudomonas sp. phDV1, in the presence of monocyclic aromatic pollutants.

Language

Greek

Subject

Aromatic compounds

Biodegradation

Complexes

ESI

MALDI

Proteomic analysis

Pseudomonas sp. phDV1

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