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Identifier |
000377911 |
Title |
Η ανίχνευση της έκφρασης των γονιδίων RKIP και RAF (A,B και C) σε ασθενείς με καρκίνο ουροδόχου κύστης |
Alternative Title |
Implication of RAF and RKIP genes in urinary bladder cancer |
Author
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Χατζηιωάννου, Μαρία Ν
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Thesis advisor
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Σπαντίδος, Δημήτριος
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Reviewer
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Σοφράς, Φραγκίσκος
Τσατσάνης, Χρήστος
Σαμώνης, Γεώργιος
Ζώρας, Οδυσσέας
Σουρβίνος, Γεώργιος
Ζαφειρόπουλος, Α.
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Abstract |
RKIP has been shown to regulate the RAS-RAF-MEK-ERK kinase cascade acting
as modulator of apoptosis and metastasis in prostate cancer. Our goal was to
examine the expression of the RAF (A-RAF, B-RAF and RAF-1) and RKIP genes
in urinary bladder cancer. Microarray analysis and qPCR was employed to
investigate the expression of RAF and RKIP, in 30 patients with transitional cell
carcinoma (TCC) of the urinary bladder vs. the corresponding levels of adjacent
normal tissue. Computational analysis was also performed on Gene Expression
Omnibus (GEO) datasets, to unravel differences in the expression of RAF or
RKIP between tumor and control samples, and between superficial and muscle
invasive tumors. Microarray analysis revealed gt;2-fold expression of BRAF and
RKIP in T2, T3, grade III tumors vs. controls. B-RAF over-expression was
verified by qPCR in pT1, grade III tumors vs. their normal counterparts
(p=0.016). qPCR revealed a significant RKIP reduction in TCC vs. normal tissue
(p=0.002 and plt;0.001 for T1, grade II and Ta-T1, grade III, respectively); All
RAF genes were positively correlated among each other (A-RAF/B-RAF,
p=0.003; A-RAF/RAF-1, plt;0.001; B-RAF/RAF-1, p=0.050), whereas B-RAF
was negatively correlated with RKIP in TCC (p=0.050). Further computational
analysis revealed different expression profiles for the genes of interest, among
muscle invasive carcinomas, superficial TCCs, cystectomy specimens and normal
tissue. The reduced RKIP mRNA levels in TCC and the elevated levels of B-RAF
in pT1, grade III tumors vs. normal tissue, corroborate that these genes are
involved in the pathogenesis of urinary bladder cancer.
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Language |
Greek |
Subject |
RKIP and RAF genes |
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Real time quantitative PCR |
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Urinary Bladder Neoplasms |
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Urinary bladder cancer |
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Γονίδια RKIP και RAF |
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Ενδοσκόπηση μέσου ρινικού πόρου |
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Καρκίνος ουροδόχου κύστεως |
Issue date |
2012-07-24 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/e/7/metadata-dlib-1357807796-221780-8812.tkl
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Views |
254 |