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Identifier

000430891

Title

Μελέτη του μεταβολισμού του θείου στο φωτοσυνθετικό βακτήριο Chlorobaculum tepidum

Alternative Title

Study of sulfur metabolism of photosynthetic bacterium Chlorobaculum tepidum

Author

Λυρατζάκης, Αλέξανδρος Γ

Thesis advisor

Τσιώτης, Γεώργιος

Reviewer

Γανωτάκης, Δημήτριος
Παυλίδης, Ιωάννης

Abstract

Chlorobaculumtepidum is an anaerobic green sulfur bacterium, which oxidizes sulfide,elemental sulfur, and thiosulfate for photosynthetic growth. This anoxygenic phototroph can oxidized sulfide to produce extracellular S⁰ globules, which can be further oxidized to sulfate. The extracellular S⁰ globules have been isolated and analyzed by MALDI-TOF MS and scanning electron microscopy (SEM). Further, to gain insight to the sulfur metabolism, the proteome of C. tepidumwas sampled at the time of the maximum sulfur production and in the stationary growth conditions and quantified using a label free quantitative proteomics strategy. In total, 1132 proteins were identified; this represents around 50% of the total proteome and more than 990 were included for thelabel-free quantification. Of these, 130 had statistically significant change to their regulation, at the point of the highest sulfur production of the strain. Cellshave an increased abundance of the periplasmicthiosulfate-oxidizing SOX enzymesand the dissimilatorysulfite reductase subunits. Finally based on the previous results we propose a model showing the regulation of sulfur metabolism proteins.

Language

Greek

Subject

Biostatistics

Mass spectrometry

Proteomics

Scanning Electron Microscopy

Βιοστατιστική

Ηλεκτρονική μικροσκοπία σάρωσης

Πρωτεωμική ανάλυση

Φασματομετρία μάζας

Issue date